pcDNA3.1/Hygro(-) 载体

基本信息

质粒类型: 哺乳细胞表达载体
启动子: CMV
克隆方法: 多克隆位点，限制性内切酶
载体大小: 5596 bp (查看载体序列)
5' 测序引物: T7 Fwd:5'-TAATACGACTCACTATAGGG-3'
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Hygromycin (潮霉素)

订购信息

产品编号 产品名称 规格 价格
VT1980 pcDNA3.1/Hygro(-) 2ug 点击询价

质粒图谱

pcDNA3.1/Hygro(-) 质粒图谱

载体描述

This pcDNA™3.1/Hygro(-) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Hygromycin selectable marker and a reverse-orientation multiple cloning site.

The pcDNA™3.1 Expression Vector Family
Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:

  • Cytomegalovirus (CMV) enhancer-promoter for high-level expression
  • Large multiple cloning site in either forward (+) or reverse (-) orientations
  • Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
  • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)
  • Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

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