质粒类型: | 哺乳细胞表达载体 |
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高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5130 bp (查看载体序列) |
5' 测序引物及序列: | T7-F:5'd[TAATACGACTCACTATAGGG]3' |
载体标签: | 6X His, Xpress |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Zeocin (博莱霉素) |
pcDNA™4/HisMax is specifically designed to maximize protein expression in a variety of mammalian cells. The vector contains the QBI SP163 translational enhancer to increase expression levels two- to five-fold above those seen with the promoter alone (Figure 1). In addition to SP163-enhanced expression, pcDNA™4/HisMax includes a cleavable N-terminal Xpress™ tag for rapid detection of recombinant protein with an Anti-Xpress™ Antibody (Figure 2). pcDNA™4/HisMax is available TOPO® Cloning-ready for five-minute cloning of Taqamplified PCR products.