质粒类型: | 四环素调控系统 |
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启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 6662bp (查看载体序列) |
5' 测序引物及序列: | pCAG-F; Bglob-intron-F |
3' 测序引物及序列: | EBV-rev; BGH-rev |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Blasticidin |
The pcDNA™6⁄TR vector is part of the T-REx™ System, which yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known.
The regulatory vector, pcDNA™6⁄TR, is provided for high-level expression of the tetracycline repressor (TR) protein. This vector expresses the Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express the TR protein.