质粒类型: | RNAi载体 |
---|---|
高拷贝/低拷贝: | 低拷贝 |
启动子: | U6 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 6486bp (查看载体序列) |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Puromycin(嘌呤霉素) |
备注: | 逆病毒基因沉默载体,能够将发卡的siRNA转染进入大多数哺乳细胞中。 |
pSilencer 5.1 Retro vectors harness retroviruses’ ability to stably integrate into the host genome of nearly all mitotically active cells and include a puromycin resistance gene for selection of cells that express the recombinant DNA. The pSilencer 5.1 Retro vectors enable researchers to study the long-term effects of reducing the expression of specific genes in cell culture models. This technology expands the capabilities of RNA interference research to address long-term alteration of gene expression.