质粒类型: | RNAi载体 |
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启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4944bp (查看载体序列) |
5' 测序引物及序列: | 5'-AGGCGATTAAGTTGGGTA-3' |
3' 测序引物及序列: | 5'-CGGTAGGCGTGTACGGTG-3' |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Neomycin (新霉素) |
The pSilencer 4.1-CMV siRNA Expression vectors are linearized with both BamH 1 and Hind III to facilitate directional cloning. They are also purified to remove the digested insert so that it cannot re-ligate with the vector. This greatly increases the percentage of clones bearing the hairpin siRNA template insert after ligation, reducing the time and effort required to screen clones.