质粒类型: | RNAi载体 |
---|---|
启动子: | H1 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4285 bp (查看载体序列) |
5' 测序引物及序列: | M13F(-40): 5'-GTTTTCCCAGTCACGAC-3' |
3' 测序引物及序列: | 3.0rev: 5'-GAGTTAGCTCACTCATTAGGC-3' |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Neomycin (新霉素) |
The pSilencer vectors employ RNA polymerase III (pol III) promoters which generate large amounts of small RNA using relatively simple promoter and terminator sequences. They also include an antibiotic resistance gene that provides a mechanism to select for transfected cells that express the introduced DNA.