质粒类型: | 哺乳动物表达载体 |
---|---|
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4679 bp (查看载体序列) |
5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG ; hGH-pA-R: CCAGCTTGGTTCCCAATAGA |
载体标签: | FLAG (N terminal) |
载体抗性: | Ampicillin (氨苄青霉素) |
备注: | shuttle vector for intracellular transient expression of N-terminal Met-FLAG |
The pFLAG-CMV-2 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG fusion proteins in mammalian cells.
The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG® -fusion constructs.
pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. SV40 replication origin results in most efficient replication in COS cells. The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG-BAP constructs.
pFLAG-CMV-2-BAP Control Plasmid is a shuttle vector for E. coli and mammalian cells. SV40 replication origin results in most efficient replication in COS cells.