质粒类型: | 慢病毒载体 |
---|---|
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 8770 bp (查看载体序列) |
5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG(Invitrogen) |
载体标签: | N-PAmCherry |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Puromycin (嘌呤霉素) |
备注: | 载体能够表达N端PAmCherry荧光蛋白 |
pLVX-PAmCherry-C1 Vector is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to infect cells and express your gene of interest fused to PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm.
Genes cloned into the MCS will be expressed as fusions to the C-terminus of PAmCherry if they are in the same reading frame as PAmCherry and there are no intervening stop codons.