质粒类型: | 慢病毒载体 |
---|---|
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 9051 bp (查看载体序列) |
5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Neomycin(新霉素) |
备注: | 编码Tet-On 3G反式激活物蛋白因子,诱导基因表达。 |
pLVX-Tet3G is a regulator vector that stably expresses the Tet-On® 3G transactivator protein, as part of any Lenti-X™ Tet-On 3G Inducible Expression System. The Lenti-X Tet-On 3G Inducible Expression Systems allow for the lentiviral delivery and inducible expression of your gene of interest in a wide variety of mammalian cells. Target cells that express the Tet-On 3G transactivator protein from pLVX-Tet3G and contain a gene of interest under the control of a pTRE3G promoter will express high levels of your gene, only when cultured in the presence of doxycycline.