质粒类型: | 腺相关病毒系统 |
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表达水平: | 高 |
启动子: | CMV |
载体大小: | 5.3 kb |
3‘ 端测序引物: | Beta-globin-F:ATTCTGAGTCCAAGCTAGGC |
5‘ 端测序引物: | hGH-PA-R: CCAGCTTGGTTCCCAATAGA |
载体抗性: | Ampicillin (氨苄青霉素) |
The pAAV-hrGFP is a nice alternative to the pAAV-LacZ positive control vector. This vector allows the you to determine the titer (i.e. the number of infectious virus particles per unit volume) of virus preparations, and confirm the transfection efficiency of their packaging cell line in vivo using the humanized Renilla GFP (hrGFP) reporter gene. This reporter gene allows for easy detection of expression in vivo using fluorescence microscopy, fluorescence-activated cell sorting (FACS) analysis.