质粒类型: | 腺相关病毒系统 |
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表达水平: | 高 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4650 bp (查看载体序列) |
5' 测序引物及序列: | CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3' |
3' 测序引物及序列: | hGH-PA-R: CCAGCTTGGTTCCCAATAGA |
载体抗性: | Ampicillin (氨苄青霉素) |
The pAAV-MCS vector contains the CMV promoter and other elements for high-level gene expression in mammalian cells when a gene of interest is cloned into the multiple cloning site (MCS). This vector contains AAV-2 inverted terminal repeats (ITRs), which direct viral replication and packaging.