pET-47b(+) 载体

基本信息

质粒类型: 大肠杆菌表达载体
表达水平: 高
克隆方法: 多克隆位点，限制性内切酶
载体大小: 5203 bp (查看载体序列)
5' 测序引物: T7
5' 测序引物序列: T7: 5'-TAATACGACTCACTATAGGG-3'
3' 测序引物: T7t
3' 测序引物序列:   T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'
载体标签: N-His, C-S, C-Thrombin
载体抗性: Kanamycin (卡那霉素)
备注:
Contains HRV 3C Protease cleavage site for fusion tag removal a low temperatures; Cterm thrombin cleavage site

订购信息

产品编号 产品名称 规格 价格
VT1916 pET-47b(+) 2ug 点击询价

质粒图谱

pET-47b(+) 质粒图谱
pET-47b(+) 载体图谱

载体描述

The pET-47b(+) plasmid features the ability to express fusion proteins with an N-terminal His•Tag® coding sequence that is cleavable with the human rhinovirus (HRV) 3C protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the HRV 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification.

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