别名: | pET14b, pet 14b |
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质粒类型: | 大肠杆菌表达载体 |
表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4671bp (查看载体序列) |
5' 测序引物: | T7 |
5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
载体标签: | N-His, N-thrombin |
载体抗性: | Ampicillin (氨苄青霉素) |
备注: |
Hosts: E.coli. Related vectors: pBR322.
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The pET-14b vector carries an N-terminal His•Tag® sequence followed by a thrombin site and three cloning sites. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.