质粒类型: | 酿酒酵母表达载体 |
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表达水平: | 高拷贝 |
诱导方法: | 半乳糖 |
启动子: | GAL1 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5963 bp (查看载体序列) |
5' 测序引物及序列: | GAL1-F: AATATACCTCTATACTTTAACGTC |
3' 测序引物及序列: | CYC1-R: GCGTGAATGTAAGCGTGAC |
载体标签: | C-His, C-V5 |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | URA3 |
备注: | 利用半乳糖诱导蛋白在酿酒酵母中表达。 |
pYES2/NT and pYES2/CT are S. cerevisiae expression vectors derived from the parental pYES2 vector. Both vectors offer the URA3 gene for selection in yeast. pYES2/NT features an N-terminal Xpress™ epitope for detection with Invitrogen's Anti-Xpress™ Antibody and a polyhistidine (6xHis) tag for purification with nickel-chelating resin. pYES2/CT contains a C-terminal V5 epitope for detection and a polyhistidine (6xHis) tag for purification.