质粒类型: | 慢病毒载体 |
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载体大小: | 12292 bp (查看载体序列) |
5' 测序引物及序列: | CMV Fwd |
3' 测序引物及序列: | SP6 rev |
载体抗性: | Ampicillin (氨苄青霉素) |
CMV promoter is upstream of the bicistronic coding cassette with SV40-Large T and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised.