pLOX-TERT-iresTK 载体
| 质粒类型: | 慢病毒载体 |
|---|---|
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 13117 bp (查看载体序列) |
| 5' 测序引物及序列: | CMV fwd: CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | SP6: ATTTAGGTGACACTATAG |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 筛选标记: | Zeocin（博来霉素） |
| 备注: | 克隆菌株为Stbl3和HB101。 |
CMV promoter is upstream of the bicistronic coding cassette with hTert and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised.
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