质粒类型: | 慢病毒载体 |
---|---|
高拷贝/低拷贝: | 低拷贝 |
启动子: | TET |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 8226 bp (查看载体序列) |
5' 测序引物及序列: | EGFP-C Forward: CATGGTCCTGCTGGAGTTCGTG |
载体标签: | GFP |
载体抗性: | Ampicillin (氨苄青霉素) |
TET promoter (minimal CMV promoter containing seven upstream Tet-operator sites) transcribes GFP and miR30-based shRNA targeting FF3. The FF3 hairpin can be replaced with any other hairpin by cloning into the EcoR1/Xho1 site.
Please note that the full plasmid sequence shown here is for the empty vector only and does not contain the FF3 targeting sequence (AGCTCCCGTGAATTGGAATCCTAGTGAAGCCACAGATGTAGGATTCCAATTCAGCGGGAGCC)