别名: | pQE81L, pQE 81L |
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质粒类型: | 大肠杆菌表达载体 |
表达水平: | 低拷贝 |
启动子: | T5 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4753 bp (查看载体序列) |
5' 测序引物及序列: | pQE30-F: AGCGGATAACAATTTCACACAG |
3' 测序引物及序列: | pQE30-R: TTCTGAGGTCATTACTGGATC |
载体标签: | N-His |
载体抗性: | Ampicillin (氨苄青霉素) |
QIAexpress pQE vectors and constructs can be maintained in any E. coli strain that is ampicillin-sensitive and carries the pREP4 repressor plasmid, or harbors the lacIq gene on the F-factor episome. Alternatively, the pQE-80L series of expression vectors which encodes a lacIq repression module, allows use of any E. coli host strain. 配套表达菌株为M15[pREP4].