别名: | pQE-80 |
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质粒类型: | 大肠杆菌表达载体 |
启动子: | T5 |
载体大小: | 4751 bp (查看载体序列) |
载体标签: | 6xHis, Nterm, lacIq |
载体抗性: | Ampicillin (氨苄青霉素) |
QIAexpress pQE vectors and constructs can be maintained in any E. coli strain that is ampicillin-sensitive and carries the pREP4 repressor plasmid, or harbors the lacIq gene on the F-factor episome. Alternatively, the pQE-80L series of expression vectors which encodes a lacIq repression module, allows use of any E. coli host strain. Promoter primer: 5'-CCCGA AAAGT GCCAC CTG-3'; Type III/IV primer: 5'-CGGAT AACAA TTTCA CACAG-3'; Reverse primer: 3'-GGTCA TTACT GGAGT CTTG-5'