质粒类型: | 昆虫细胞表达载体 |
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高拷贝/低拷贝: | 高拷贝 |
启动子: | OpIE2 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 3336bp (查看载体序列) |
5' 测序引物及序列: | OpIE2:5'd[CGCAACGATCTGGTAAACAC]3' |
载体标签: | V5 (Cterm), His (Cterm) |
载体抗性: | Zeocin (博莱霉素) |
筛选标记: | Zeocin |
备注: | Stable expression of gene in insect cells. GFP reporter allows visualization of stable cells. |
The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the following features:
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistanceprotein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lineswith Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.