质粒类型: | 哺乳动物表达载体 |
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启动子: | UbC |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5463 bp (查看载体序列) |
5' 测序引物及序列: | T7 Fwd:5'd[TAATACGACTCACTATAGGG]3' |
载体标签: | His Tag (6x), V5 Epitope Tag |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Blasticidin |
All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.