pMIB/v5-His B 载体

基本信息

质粒类型: 昆虫细胞表达载体
高拷贝/低拷贝: 高拷贝
启动子: OpIE2
克隆方法: 多克隆位点，限制性内切酶
载体大小: 3600bp (查看载体序列)
5' 测序引物及序列: OpIE2-F: 5'd[CGCAACGATCTGGTAAACAC]3'
载体标签: HBM (Nterm), V5 (Cterm), His (Cterm)
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Blasticidin
备注: 在昆虫细胞中能够稳定表达，HBM标签可实现目的蛋白的分泌表达

订购信息

产品编号 产品名称 规格 价格
VT1176 pMIB/v5-His B 2ug 点击询价

质粒图谱

pMIB/v5-His A,B,and C 质粒图谱

载体描述

载体描述

pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:

  • The OpIE2 promoter for constitutive expression
  • The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
  • The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
  • C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin

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