pAc5.1/V5-His A 载体

基本信息

别名: pAc5.1/V5-HisA
质粒类型: 果蝇细胞表达载体
启动子: pAC5
克隆方法: 多克隆位点，限制性内切酶
载体大小: 5375 bp (查看载体序列)
5' 测序引物及序列: AC5:5'd[ACACAAAGCCGCTCCATCAG]3'
载体标签: V5 Epitope Tag, His Tag (6x)
载体抗性: Ampicillin (氨苄青霉素)
备注:
Constitutive insect cell expression. Can use pCoHygro or pCoBlast for selection.

订购信息

产品编号 产品名称 规格 价格
VT1087 pAc5.1/V5-His A 2ug 点击询价

质粒图谱

pAc5.1/V5-His A, B, C 质粒图谱

载体描述

The DES®-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. The vector offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAc5.1/V5-His vector also offers the following features:

  • Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency
  • C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
  • C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.

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