质粒类型: | Cre/Lox系统 |
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载体大小: | 6678 bp (查看载体序列) |
载体抗性: | Ampicillin |
筛选标记: | Puromycin |
备注: | This vector was designed for assembling gene targeting constructs using the BAC recombineering approach. The vector contains a puromycin-(delta)thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pUDTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo). These selectable markers are flanked by lox66 and lox2272 sites (Cre-recombinase recognition sequences) in a pBluescript KS+ backbone with a modified multi-cloning region |