pEGM-7ZF(+) 载体

质粒类型: 大肠杆菌克隆载体
克隆方法: 多克隆位点,限制性内切酶
载体大小: 2997 bp (查看载体序列)
载体抗性: Ampicillin (氨苄青霉素)
产品编号 产品名称 规格 价格
VT2058 pEGM-7ZF(+) 2ug 点击询价

The pGEM ® -7Zf(+) Vector (a) is a derivative of the pGEM ® -3Zf(+) Vector (a) and con- tains the origin of replication of the filamentous phage f1. The plasmid serves as a standard cloning vector, as a template for in vitro transcription, and can be used for the production of circular ssDNA. The plasmid contains SP6 and T7 RNA poly- merase promoters flanking a region of multiple cloning sites within the α -peptide coding region of β -galactosidase (1). Insertional inactivation of the α -peptide allows recombinant clones to be directly identified by color screening on indicator plates. The multiple cloning region is unique and includes restriction sites for Apa I, Aat II, Sph I, Xba I, Xho I, Eco R I, Kpn I, Sma I, Csp 45 I, Cla I, Hin d III, Bam H I, Sac I, Bst X I and Nsi I. This arrangement is designed specifically for generation of unidirec- tional deletions with Promega’s Erase-a-Base ® System. The polylinker contains restriction enzyme sites that produce 5 ́ overhangs or blunt ends (sensitive to Exonuclease III) flanked on both sides by blocks of restriction sites that generate 3 ́ overhangs (resistant to Exonuclease III).