质粒类型: | 慢病毒载体 |
---|---|
高拷贝/低拷贝: | 高拷贝 |
启动子: | TetO |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 13320bp (查看载体序列) |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Puromycin(嘌呤霉素) |
The Thermo Scientific™ TRIPZ™ Inducible Lentiviral shRNA Collection was developed in collaboration with Dr. Greg Hannon of Cold Spring Harbor Laboratory (CSHL) and Dr. Steve Elledge of Harvard Medical School. This collection combines the design advantages of microRNA-adapted short hairpin RNA (shRNA) with the pTRIPZ lentiviral inducible vector to create a powerful RNAi trigger capable of producing gene silencing. The vector is engineered to be Tet-On and produces tightly regulated induction of shRNA expression in the presence of doxycycline.