别名: | pPink(alpha)-HC |
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质粒类型: | 酵母表达载体 |
表达水平: | 低拷贝 |
启动子: | AOX1 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 7898 bp (查看载体序列) |
5' 测序引物及序列: | 5´ AOX1:5´-GACTGGTTCCAATTGACAAGC-3´ alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´ |
3' 测序引物及序列: | 3´ AOX1:5´-GCAAATGGCATTCTGACATCC-3´ |
载体标签: | N-alpha 分泌信号肽 |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | ADE2 |
备注: | 拥有alpha因子,分泌表达蛋白 |
The PichiaPink™ Secreted Vector Kit contains the pPINKα-HC vector for use with the PichiaPink™ Yeast Expression System. The pPINKα-HC vector is built around the ADE2 gene for complementing the complementing the ade2 deletion in the PichiaPink™ strains. The pPINKα-HC vector has the α-mating factor secretion signal sequence so that all proteins are secreted. You can order the PichiaPink™ Secreted Vector Kit to refill your PichiaPink™ Yeast Expression System Kit.