质粒类型: | 慢病毒载体 |
---|---|
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 7032 bp (查看载体序列) |
5' 测序引物及序列: | GACTATCATATGCTTACCGT |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Puromycin |
pLKO.1-puro is a transfer and cloning vector of shRNA, originally derived from HIV-1. It contains all the necessary cis-elements for packaging, reverse transcription, and integration, which are required for subsequent production of the lentiviral particles.
AgeI and EcoRI (only these two sites available)