质粒类型: | 启动子报告载体 |
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克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4370 bp (查看载体序列) |
载体抗性: | Ampicillin (氨苄青霉素) |
备注: | Promoterless vector encoding destabilized luciferase for measuring the activity of promoter and enhancer sequences. |
The pGL4.11[luc2P] Vector(a–d)encodes the luciferase reporter gene luc2P (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.
The pGL4.11[luc2P] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice. The luc2P reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.