质粒类型: | Cytotrap Two-Hybrid System |
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启动子: | ADH1 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 11.3kb |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | LEU2 |
The pSos vector are designed for constructing and expressing gene fusions with the hSos protein and a myristylation signal, respectively. The pSos vector contains DNA encoding amino acids (aa) 1 to 1067 of the hSos gene and unique 3´ cloning sites.2 It is used for constructing a bait plasmid containing a DNA insert encoding a bait protein. The ADH1 promoter driving expression of the hSos-bait fusion is constitutively active