GenBank序列号: | AF334399 |
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质粒类型: | 腺病毒系统 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | CMV |
载体大小: | 6568 bp (查看载体序列) |
5' 测序引物及序列: | pShuttle-F: GAAGTGAAATCTGAATAATTTTGTG |
3' 测序引物及序列: | pShuttle-R: CAAAACTACATAAGACCCCCAC |
载体抗性: | Kanamycin (卡那霉素) |
pShuttle contains only a multiple cloning site. This allows for the insertion of an entire expression cassette, including specialized promoters and termination signals (up to 7.5 kb). The regions indicated as arms are the stretches of sequence homology with pAdEasy-1 where the homologous recombination occurs. The R-ITR and L-ITR regions are short inverted terminal repeats (Left and Right) which have a role in replication of the viral DNA.