质粒类型: | 大肠杆菌表达载体 |
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高拷贝/低拷贝: | 低拷贝 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4245bp (查看载体序列) |
载体抗性: | Chloramphenicol(氯霉素) |
筛选标记: | Tetracycline (四环素) |
pACYC184 can be used together with, or as an alternative multipurpose vector to, pBR322. The origin of replication of pACYC184 is derived from the miniplasmid p15A1, whereas that of pBR322 and its derivatives is from pColE. These origins are compatible and pACYC184 and pBR322 can therefore co-exist within the same cell. This allows two recombinant plasmids to be maintained and expressed simultaneously.