pETDuet-1 载体

基本信息

质粒类型: 大肠杆菌表达载体（双启动子）
表达水平: 高
克隆方法: 多克隆位点，限制性内切酶
载体大小: 5420 bp (查看载体序列)
5' 测序引物及序列: T7: 5'-TAATACGACTCACTATAGGG-3';
3' 测序引物及序列:   T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'
载体标签: N-His, C-S
载体抗性: Ampicillin (氨苄青霉素)
备注:
pETDuet-1 is designed for the coexpression of two target genes. pETDuet-1 carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene.

订购信息

产品编号 产品名称 规格 价格
VT1237 pETDuet-1 2ug 点击询价

质粒图谱

pETDuet-1质粒图谱
pETDuet-1载体图谱

载体描述

pETDuet-1 is designed for the coexpression of two target genes. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and a ribosome binding site (rbs). The vector also carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene. This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3) in an appropriate host strain for the coexpression of up to 4 target genes. Genes inserted into MCS1 can be sequenced using the pET Upstream Primer (Cat. No. 69214-3) and DuetDOWN1 Primer (Cat. No. 71179-3). Genes inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3).