质粒类型: | 昆虫细胞表达载体 |
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高拷贝/低拷贝: | 高拷贝 |
启动子: | Polyhedrin |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4853 bp (查看载体序列) |
5' 测序引物及序列: | polyhedrin forward |
载体标签: | His |
载体抗性: | Ampicillin (氨苄青霉素) |
pBlueBacHis2 A (4863 bp), pBlueBacHis2 B (4853 bp), and pBlueBacHis2 C (4861 bp) are baculovirus transfer vectors that contain the Xpress N-terminal tag for expression and purification of recombinant fusion proteins. The pBlueBacHis2 vector is provided in three different versions for simplified cloning of your gene in-frame with the Xpress tag. The vectors contain sequences homologous to the lacZ gene and ORF1629 sequences in Bac-N-Blue linear DNA, allowing production of blue, recombinant plaques. Expression of your recombinant fusion protein is driven by the polyhedrin promoter.