质粒类型: | FRET assay 载体 |
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载体大小: | 3348 bp (查看载体序列) |
5' 测序引物及序列: | M13 fwd |
3' 测序引物及序列: | M13 rev |
载体抗性: | Ampicillin (氨苄青霉素) |
This plasmid contains the coding sequence of a dimeric vesion of the fluorescent protein “Keima-Red,” which was cloned from the stony coral whose Japanese name is“Komon-Sango.” CoralHue® dKeima-Red absorbs light maximally at 440 nm and emits red light at 616 nm. Thus CoralHue® dKeima-Red exhibits an extremely large Stokes shift (176 nm). The red fluorescence is stable under usual aerobic conditions. The combination of red emission, a very large Stokes shift, stability at 37℃ in eukaryotic cells, and being dimeric make dKeima-Red a superb reporter protein for labeling proteins or subcellular structures in multicolor fluorescence analyses.