质粒类型: | 荧光蛋白报告载体 |
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启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4722 bp (查看载体序列) |
5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | mCherry-R: TTGGTCACCTTCAGCTTGG |
载体标签: | mCherry |
载体抗性: | Kanamycin (卡那霉素) |
筛选标记: | Neomycin |
mCherry is a mutant fluorescent protein derived from the tetrameric Discosoma sp. red fluorescent protein DsRed.(excitation and emission maxima of native mCherry protein are 587nm and 610nm. Genes of interest can be cloned into the MCS and will be expressed as fusions to the N-terminus of mCherry (source: Clontech). transgenic expression of mCherry-tagged proteins in mammalian cells