pBudCE4.1 载体

基本信息

质粒类型: 哺乳动物表达载体(双启动子)
启动子: EF-1α, CMV
克隆方法: 多克隆位点，限制性内切酶
载体大小: 4595 bp (查看载体序列)
5' 测序引物及序列: CMVPro-F: 5'd[TAATACGACTCACTATAGGG]3'
EF1aPro-F:5'd[TCAAGCCTCAGACAGTGGTTC]3'
载体标签: 6xHis, c-Myc, V5
载体抗性: Zeocin (博莱霉素)
筛选标记: Zeocin (博莱霉素)
备注: 能够利用该载体同时表达两种基因

订购信息

产品编号 产品名称 规格 价格
VT1019 pBudCE4.1 2ug 点击询价

质粒图谱

pBudCE4.1 质粒图谱

载体描述

The pBudCE4.1 vector is designed for the independent expression of two genes from a single plasmid in mammalian cells. Using pBudCE4.1 to generate stable mammalian expression cell lines ensures that there is an equivalent copy number of each gene in the cell. This can eliminate variable expression due to differences in gene copy number. pBudCE4.1 provides expression cassettes withthe following features:

  • The CMV promoter for high-level transcription of genes with an optional c-myc epitope tag for rapid detection and 6xHis sequence for simple purification
  • The human EF-1α promoter for high-level expression of genes with an optional V5 epitope tag for rapid detection and 6xHis sequence for simple purification
  • The Sh ble (ZeoR) gene for efficient selection in both mammalian cells and E. coliwith the selection agent Zeocin™