测序通用引物全搜集
2014-07-23
相信大家在送DNA测序的时候一般都要求填写载体的名称，如果是T载体或pcDNA3这种常见载体当然没有问题。但如果不是常用的载体，就需要自己合成引物(需要填写序列)或者指定一个通用引物的名称。实际上，我们在遇到后面一种情况时，可以分析下自己载体上有没有下面列举的常用元件。如果有，可以比较下后面列出的序列是否与你的载体序列匹配(这个是以防万一名称相同但序列却有差异)，如果也是匹配的，就可以写上这些通用引物的名称（最好在下面也附上相应的序列，防止有些时候测序公司的通用引物库不全）。这样既可以节约自己合成引物的花费，而且这些引物的序列都是经反复优化的，可能比我们自己设计的序列效果更好！
下面是我们从网上搜集的最全的测序通用引物列表：
测序通用引物列表
| 引物名称 | 序列(5'→3') | 描述 |
|---|---|---|
| 3'AOX1 | GCAAATGGCATTCTGACATCC | For Pichia vectors with AOX1 terminator, reverse primer |
| 5'AOX1 | GACTGGTTCCAATTGACAAGC | For Pichia vectors with AOX1 promoter, forward primer |
| Polyhedrin forward | AAATGATAACCATCTCGC | Polyhedrin promoter, forward primer |
| pGP704-R | AACAAGCCAGGGATGTAACG | R6K gamma origin, 3' of MCS in pGP704 vector, reverse primer |
| SeqL-B (ATTL2) | AACATCAGAGATTTTGAGACAC | |
| BAC1 | AACCATCTCGCAAATAAATA | |
| PIRES3-EGFP-R | AACGCACACCGGCCTTATTC | |
| Ubx-F | AACTCGTACTTTGAACAGGC | Drosophila Ultrabithorax gene, forward primer |
| PBV220F | AAGAAGGGCAGCATTCAAAG | |
| pbd-gal4-r | AAGAGTTACTCAAGAACAAGAA | |
| pMRB101-F | AAGATGCAGGCAGCTGAGTT | HCMV major immediate-early protein (IE), forward primer |
| PB42AD-R | AAGCCGACAACCTTGATTGGAG | |
| T7EEV | AAGGCTAGAGTACTTAATACG | |
| Gal4-AD | AATACCACTACAATGGAT | 3' end of Gal4 activation domain, forward primer |
| GAL4-ADfor | AATACCACTACAATGGATGATG | 3' end of Gal4 activation domain, forward primer |
| pBRforEco | AATAGGCGTATCACGAGGC | In pBR322, upsteam of EcoRI site, forward primer |
| GAL1 | AATATACCTCTATACTTTAACGTC | S. cerevisiae GAL1 promoter, forward primer |
| NOSR | AATCATCGCAAGACCGGC | |
| GW-5' | AATCTCGCCGGATCCTAACT | 5' end of Gateway cassette, reverse primer |
| AC5 | ACACAAAGCCGCTCCATCAG | Drosophila Actin 5C promoter, forward primer |
| T7(17base) | ACATCCACTTTGCCTTTCTC | |
| RCAS-F | ACATGGGTGGTGGTATAGCGCTTGCG | 3' of Rous sarcoma virus (RSV) env gene, forward primer |
| GST-Tag | ACCCAATGTGCCTGGATGCG | |
| pBABE 3' | ACCCTAACTGACACACATTCC | SV40 enhancer, 3' of MCS in pBABE vectors, reverse primer |
| V5 Reverse | ACCGAGGAGAGGGTTAGGGAT | V5 epitope, reverse primer |
| pLNCX-R | ACCTACAGGTGGGGTCTTTCATTCCC | |
| BAC2 | ACGCACAGAATCTAGCGCTT | |
| pLTet-F | ACTGAGCACATCAGCAGGAC | Lambda phage early leftward (pL) promoter, forward primer |
| pCEP-F | AGAGCTCGTTTAGTGAACCG | |
| F27 | AGAGTTTGATCCTGGCTCA | |
| 3'AD | AGATGGTGCACGATGCACAG | |
| L4440 | AGCGAGTCAGTGAGCGAG | 5' of MCS in L4440 vector, forward primer |
| M13 Reverse (-48) | AGCGGATAACAATTTCACAC | |
| M13/pUC Reverse | AGCGGATAACAATTTCACACAGG | In lacZ gene |
| M13R(-48) | AGCGGATAACAATTTCACACAGGA | In lacZ gene |
| LNCX | AGCTCGTTTAGTGAACCGTCAGATC | Human CMV promoter, forward primer |
| pLNCX-F | AGCTCGTTTAGTGAACCGTCAGATCG | |
| DsRed1-C | AGCTGGACATCACCTCCCACAACG | 3' end of DsRed1, forward primer |
| 2.0rev primer | AGGCGATTAAGTTGGGTA | |
| CMV-Reverse | AGTAGGAAAGTCCCGTAAGG | |
| Luc-F | AGTCAAGTAACAACCGCGA | 3' end of luciferase, forward primer |
| N-CMV-30 | ATAACCCCGCCCCGTTG | |
| Amp-R | ATAATACCGCGCCACATAGC | 5' end of ampicillin resistance gene, reverse primer |
| MMLV-F | ATCAGTTCGCTTCTCGCTTC | Moloney murine leukemia virus LTR (MoMuLV), forward primer |
| pBAD Forward | ATGCCATAGCATTTTTATCC | For vectors with E. coli araBAD promoter, forward primer |
| pET-Upstream | ATGCGTCCGGCGTAGA | |
| U6 | ATGGACTATCATATGCTTACCGTA | |
| pENTR-R | ATGGCTCATAACACCCCTTG | 3' of attL2 in pENTR vector, reverse primer |
| T3 | ATTAACCCTCACTAAAGGGA | T3 promoter, forward primer |
| pCMV5R | ATTATAGAGGACACCTAGTC | |
| pDsRed-C-3' | ATTCATTTTATGTTTCAGGTTCA | |
| SFFV-F | ATTGATTGACTGCCCACCTC | Spleen focus forming virus 5' LTR, forward primer |
| SP6 | ATTTAGGTGACACTATAG | SP6 promoter, forward primer |
| S1 | CAACGTGAAAAAATTATTATTCGC | |
| TRC-F | CAAGGCTGTTAGAGAGATAATTGGA | Human U6 promoter, forward primer |
| T3 | CAATTAACCCTCACTAAAGG | |
| AUG1 Forward | CAATTTACATCTTTATTTATTAACG | For Pichia vectors with AUG1 promoter, forward primer |
| SV40-spliceR | CACAAAGATCCGGACCAAAG | SV40 splice sequence, reverse primer |
| pIRES.R | CACTGCATTCTAGTTGTGGT | |
| mU6-F | CAGCACAAAAGGAAACTCACC | Mouse U6 promoter, forward primer |
| MSCV-rev | CAGCGGGGCTGCTAAAGCGCATGC | Murine stem cell virus, reverse primer |
| M13 Reverse | CAGGAAACAGCTATGAC | In lacZ gene |
| M13R | CAGGAAACAGCTATGACC | |
| mU6 | CAGTGCAGGGGAAAGAATAGTAGAC | |
| WPRE-R | CATAGCGTAAAAGGAGCAACA | 5' end of WPRE, reverse primer |
| MT Forward | CATCTCAGTGCAACTAAA | Drosophila metallothionein promoter, forward primer |
| EGFP-C | CATGGTCCTGCTGGAGTTCGTG | 3' end of EGFP, forward primer |
| mPGK-F | CATTCTGCACGCTTCAAAAG | Mouse PGK promoter, forward primer |
| pTRE 3' | CCACACCTCCCCCTGAAC | |
| PB42AD-F | CCAGCCTCTTGCTGAGTGGAGATG | |
| hGH-PA-R | CCAGCTTGGTTCCCAATAGA | Human growth hormone terminator, reverse primer |
| Rluc-F | CCAGGATTCTTTTCCAATGC | 3' end of Renilla luciferase, forward primer |
| pTarget.F | CCAGGATTTTCCCAGTCAC | |
| pCAMBIA1301-5' | CCAGGCTTTACACTTTATGC | |
| GFP-R | CCATCTAATTCAACAAGAATTGGGACAAC | 5' end of GFP, reverse primer |
| M13/pUC Forward | CCCAGTCACGACGTTGTAAAACG | In lacZ gene |
| mCherry-F | CCCCGTAATGCAGAAGAAGA | 3' end of mCherry, forward primer |
| pQE promoter | CCCGAAAAGTGCCACCTG | 5' of MCS in pQE vectors, forward primer |
| Intein Forward | CCCGCCGCTGCTTTTGCACGTGAG | |
| M13(-96) | CCCTCATAGTTAGCGTAACG | |
| MSCV|pLXSN 5' | CCCTTGAACCTCCTCGTTCGACC | Murine stem cell virus, forward primer |
| pGEX 3' | CCGGGAGCTGCATGTGTCAGAGG | 3' of MCS in pGEX vectors, reverse primer |
| IRES-R | CCTCACATTGCCAAAAGACG | 5' end of IRES, reverse primer |
| SV40-pArev | CCTCTACAAATGTGGTATGG | |
| LucNrev | CCTTATGCAGTTGCTCTCC | 5' end of luciferase, reverse primer |
| S.tag | CGAACGCCAGCACATGGACA | |
| CMV Forward | CGCAAATGGGCGGTAGGCGTG | Human CMV immediate early promoter, forward primer |
| OpIE2 Forward | CGCAACGATCTGGTAAACAC | OpIE2 promoter, forward primer |
| M13F（-47） | CGCCAGGGTTTTCCCAGTCACGAC | |
| pTRE 5' | CGCCTGGAGACGCCATCC | |
| pBluescript SK | CGCTCTAGAACTAGTGGATC | |
| pRS-marker | CGGCATCAGAGCAGATTGTA | To sequence yeast selectable marker in pRS vectors |
| GPDpro-F | CGGTAGGTATTGATTGTAATTCTG | S. cerevisiae GPD promoter, forward primer |
| pLEXA-F | CGTCAGCAGAGCTTCACCAT | |
| LexA | CGTCAGCAGAGCTTCACCATTG | 3' end of LexA DNA binding domain, forward primer |
| EGFP-Nrev | CGTCGCCGTCCAGCTC | |
| EGFP-N | CGTCGCCGTCCAGCTCGACCA | |
| EGFP-N | CGTCGCCGTCCAGCTCGACCAG | 5' end of EGFP, reverse primer |
| PinPoint primer | CGTGACGCGGTGCAGGGCG | |
| Neo-F | CGTTGGCTACCCGTGATATT | 3' end of neomycin resistance gene, forward primer |
| pENTR-F | CTACAAACTCTTCCTGTTAGTTAG | 5' of attL1 in pENTR vector, forward primer |
| PCDNA3.1F | CTAGAGAACCCACTGCTTAC | |
| RVprimer3 | CTAGCAAAATAGGCTGTCCC | 5' of MCS in pGL3 vector, forward primer |
| 35S promoter | CTATCCTTCGCAAGACCCTTC | CaMV 35S promoter, forward primer |
| 5'TriplEx2 | CTCCGAGATCTGGACGAGC | |
| Ecdysone Forward | CTCTGAATACTTTCAACAAGTTAC | Drosophila heat shock promoter, forward primer |
| pFastBac-3' | CTGATTATGATCCTCTAGTACTTC | |
| PBV220R | CTGCGTTCTGATTTAATCTG | |
| Bglob-intron-F | CTGGTCATCATCCTGCCTTT | Rabbit beta-globin intron, forward primer |
| tdTomato-Fwd | CTGTTCCTGTACGGCATGG | 3' end of tdTomato, forward primer |
| pIRES-F | CTTACTGACATCCACTTTGC | |
| Pry1 | CTTAGCATGTCCGTGGGGTTTGAAT | PZ P-element, reverse primer |
| TKpolyAreverse | CTTCCGTGTTTCAGTTAGC | |
| pLXSN-F | CTTGAACCTCCTCGTTCGAC | |
| pBRforBam | CTTGGAGCCACTATCGAC | In pBR322 tet region, upstream of BamHI, forward primer |
| pBABE 5' | CTTTATCCAGCCCTCAC | Psi packaging signal, 5' of MCS in pBABE vectors, forward primer |
| GLP2 | CTTTATGTTTTTGGCGTCTTCCA | |
| SV40pA-R | GAAATTTGTGATGCTATTGC | SV40 polyA, reverse primer |
| S.tag | GAACGCCAGCACATGGAC | |
| AUG1 Reverse | GAAGAGAAAAACATTAGTTGGC | For Pichia vectors with AUG1 promoter, reverse primer |
| LacZ-R | GACAGTATCGGCCTCAGGAA | 5' end of LacZ, reverse primer |
| RVP4 | GACGATAGTCATGCCCCGCG | |
| pbi-121(35s) | GACGCACAATCCCACTATCC | |
| pMX-S1811 | GACGGCATCGCAGCTTGGATACAC | MMLV sequence, 5' of MCS in pMXs vector, forward primer |
| HU6 | GACTATCATATGCTTACCGT | |
| XBG-R | GACTCCATTCGGGTGTTC | Xenopus beta-globin 3'UTR, reverse primer |
| Tac promoter | GAGCGGATAACAATTTCACACAGG | Tac promoter, forward primer |
| HAT | GAGGAGCACGCTCATGCCCAC | Histidine affinity tag, forward primer |
| pTrcHis Forward | GAGGTATATATTAATGTATCG | 5' of MCS in pTrcHis vector, forward primer |
| Gal4 N-term | GAGTAGTAACAAAGGTCAA | 3' end of Gal4 DNA binding domain, forward primer |
| pHybLex Reverse | GAGTCACTTTAAAATTTGTATACAC | ADH terminator, reverse primer |
| 3.0rev | GAGTTAGCTCACTCATTAGGC | |
| NOS | GATAATCATCGCAAGACCGG | |
| pCMVF | GATCCGGTACTAGAGGAACTGAAAAAC | |
| DuetUP1 | GATCTCGACGCTCTCCCT | |
| MBP-F | GATGAAGCCCTGAAAGACGCGCAG | 3' end of maltose binding protein, forward primer |
| pYESTrp Forward | GATGTTAACGATACCAGCC | |
| DuetDOWN1 | GATTATGCGGCCGTGTACAA | |
| pBAD Reverse|pTrcHis Reverse | GATTTAATCTGTATCAGG | For vectors with E. coli araBAD promoter, reverse primer |
| CRE-R | GCAAACGGACAGAAGCATTT | 5' end of Cre recombinase, reverse primer |
| Puro-F | GCAACCTCCCTTCTACGAGC | 3' end of puromycin resistance gene, forward primer |
| pCAG-F | GCAACGTGCTGGTTATTGTG | Rabbit beta-globin intron, for pCAG plasmids, forward primer |
| pCasper-hs | GCAACTACTGAAATCTGCCAAG | Drosophila Hsp70 promoter, forward primer |
| CAT-R | GCAACTGACTGAAATGCCTC | 5' end of chloramphenicol resistance gene, reverse primer |
| Nmt1-F | GCAATGTGCAGCGAAACTAA | S. pombe nmt1 promoter, forward primer |
| T3 | GCAATTAACCCTCACTAAAGG | T3 promoter, forward primer |
| pFlag-CMV-R | GCACTGGAGTGGCAACTT | |
| Myc | GCATCAATGCAGAAGCTGATCTCA | Myc tag, forward primer |
| GW-3' | GCATGATGACCACCGATATG | 3' end of Gateway cassette, forward primer |
| Neo-R | GCCCAGTCATAGCCGAATAG | 5' end of neomycin resistance gene, reverse primer |
| SV40-Promoter | GCCCCTAACTCCGCCCATCC | |
| pbd-gal4-f | GCCTCTAACATTGAGACAGC | |
| SeqL-A (ATTL1) | GCGAGAGTAGGGAACTGC | |
| pCAMBIA1301-3' | GCGATTAAGTTGGGTAACGC | |
| CYC1|pYESTrp Reverse | GCGTGAATGTAAGCGTGAC | CYC1 transcription termination signal, reverse primer |
| NOS-F | GCGTTCAAAAGTCGCCTAAG | Nopaline synthase promoter, forward primer |
| pAd-CMV | GCTAGAGATCTGGTACCGTC | For cloning sites after SalI in pAd-CMV vector |
| T7 Terminal | GCTAGTTATTGCTCAGCGG | T7 terminator, reverse primer |
| pREP Forward | GCTCGATACAATAAACGCC | Rous sarcoma virus (RSV) promoter, forward primer |
| MT1-F | GCTGTCCTCTAAGCGTCACC | Mouse metallothionein 1 promoter, forward primer |
| pBMN 5' | GCTTGGATACACGCCGC | MMLV sequence, for inserts in pBMN retroviral vector |
| M13 Reverse (-27) | GGAAACAGCTATGACCATG | |
| SV40-F | GGAGGCTTTTTTGGAGGC | |
| 3'AOX | GGCAAATGGCATTCTGACAT | |
| PCDNA3.0-R2 | GGCAACTAGAAGGCACAGTC | |
| LacI-R | GGCATACTCTGCGACATCGT | 5' end of LacI, reverse primer |
| Tet-R | GGCGAGTTTACGGGTTGTTA | 5' end of tetracycline resistance gene, reverse primer |
| pTarget.R | GGCTTTACACTTTATGCTTC | |
| pBR322ori-F | GGGAAACGCCTGGTATCTTT | pBRS322 origin, forward primer |
| U6 Primer | GGGCAGGAAGAGGGCCTAT | |
| pGEX 5' | GGGCTGGCAAGCCACGTTTGGTG | 3' end of glutathione-S-transferase, forward primer |
| Tn7-end | GGGGTGGAAATGGAGTTTTT | Bacterial transposon Tn7 |
| pCasper-F | GGGTTTTATTAACTTACAT | 5' end of Drosophila mini-white gene, reverse primer |
| pFlag-CMV-F | GGTAGGCGTGTACGGTGG | |
| GFP-F | GGTCCTTCTTGAGTTTGTAAC | 3' end of GFP, forward primer |
| MalE Primer | GGTCGTCAGACTGTCGATGAAGCC | |
| pSHUTTLE-CMV-F | GGTCTATATAAGCAGAGGTG | |
| pBRrevBam | GGTGATGTCGGCGATATAGG | In pBR322 tet region, downstream of BamHI, reverse primer |
| Gal10pro-F | GGTGGTAATGCCATGTAATATG | S. cerevisiae GAL10 promoter, forward primer |
| 1492R | GGTTACCTTGTTACGACTT | |
| M13 Forward (-20) | GTAAAACGACGGCCAGTG | |
| S6 | GTAAATGAATTTTCTGTAGTAGG | |
| pPc86-3' | GTAAATTTCTGGCAAGGTAGAC | |
| DsRed1-N | GTACTGGAACTGGGGGGACAG | 5' end of DsRed1, reverse primer |
| PIRES2-EGFP-F | GTAGGCGTGTACGGTGGGAG | |
| Polyhedrin reverse | GTCCAAGTTTCCCTG | For baculovirus vector with polyhedrin promoter, reverse primer |
| pFastBac-5' | GTCCGAAACCATGTCGTAC | |
| EXFP-R | GTCTTGTAGTTGCCGTCGTC | For distinguishing EGFP vs ECFP vs EYFP, reverse primer |
| pACT2-R | GTGCACGATGCACAGTTGAA | |
| F1ori-F | GTGGACTCTTGTTCCAAACTGG | F1 origin, forward primer |
| pSHUTTLE-CMV-R | GTGGTATGGCTGATTATGATCAG | |
| EBV Reverse | GTGGTTTGTCCAAACTCATC | SV40 polyA terminator, reverse primer |
| CMV-R | GTTCACGGTGCCCTCC | |
| pQE30-R | GTTCTGAGGTCATTACTGG | |
| pcDL-F | GTTGCCTTTACTTCTAGGCCT | 5' of EcoRI site in pcDL vector, forward primer |
| pLXSN-R | GTTGCTGACTAATTGAGATG | |
| M13 (-40) | GTTTTCCCAGTCACGAC | In lacZ gene |
| pLEXA-R | TAAAACCTAAGAGTCACTTT | |
| 3'BD | TAAGAGTCACTTTAAAATTTGTA | |
| T7 | TAATACGACTCACTATAGGG | T7 promoter, forward primer |
| GAL4 AD | TACCACTACAATGGATG | |
| HA-F | TACCCATACGACGTCCCAGA | HA tag, forward primer |
| SP6 | TACGATTTAGGTGACACTATAG | |
| α-Factor | TACTATTGCCAGCATTGCTGC | |
| BGH Reverse|pcDNA3.1R | TAGAAGGCACAGTCGAGG | Bovine growth hormone terminator, reverse primer |
| pPc86F | TATAACGCGTTTGGAATCACT | |
| Xpress Forward | TATGGCTAGCATGACTGGT | Xpress epitope, forward primer |
| PEGFP-C | TATGGCTGATTATGATCAGT | |
| SV40pro-F | TATTTATGCAGAGGCCGAGG | SV40 promoter/origin, forward primer |
| EF-1a Forward | TCAAGCCTCAGACAGTGGTTC | Human elongation factor-1a promoter, forward primer |
| GAL4 BD | TCATCGGAAGAGAGTAG | |
| GAL4-BDfor | TCATCGGAAGAGAGTAGTAA | |
| pCMV-R | TCCAAACTCATCAATGTATC | |
| pDsRED-express-C1-F | TCCCACAACGAGGACTACAC | |
| hrGFP-R | TCCCCGAGTACCACTTCATC | hrGFP (humanized Renilla GFP), forward primer |
| ITS1 | TCCGTAGGTGAACCTGCGG | |
| ITS4 | TCCTCCGCTTATTGATATGC | |
| pZIP | TCCTTTCCAGCGAGGTTCTA | Murine leukemia virus (MuLV), reverse primer |
| pBluescriptKS | TCGAGGTCGACGGTATC | For pBluescript vector |
| H1 | TCGCTATGTGTTCTGGGAAA | Human H1 promoter, forward primer |
| pYes2.R | TCGGTTAGAGCGGATGTG | |
| pCMV-F | TCTAAAAGCTGCGGAATTGT | |
| pBluescriptSK | TCTAGAACTAGTGGATC | For pBluescript vector |
| HA-R | TCTGGGACGTCGTATGGGTA | HA tag, reverse primer |
| tdTomato-Rev | TCTTTGATGACGGCCATGT | 5' end of tdTomato, reverse primer |
| pDSRED-N1-R | TGAAGCGCATGAACTCCTTG | |
| hUBCpro-F | TGAAGCTCCGGTTTTGAACT | Human Ubiquitin C (UbC) promoter, forward primer |
| pACYC-F | TGAAGTCAGCCCCATACGAT | p15A origin, forward primer |
| pQE30-F | TGAGCGGATAACAATTTCAC | |
| P5'|pMAL-C2X-F | TGCGTACTGCGGTGATCAAC | |
| T7TER | TGCTAGTTATTGCTCAGCGG | |
| IRES-F | TGGCTCTCCTCAAGCGTATT | 3' end of IRES, forward primer |
| pEGFP-N-5’ | TGGGAGGTCTATATAAGCAGAG | |
| M13 (-21) Forward | TGTAAAACGACGGCCAGT | In lacZ gene |
| GLP1 | TGTATCTTATGGTACTGTAACTG | |
| C-CMV-24 | TTAGGACAAGGCTGGTGG | |
| PCMV-5F | TTCCAAAATGTCGTAATAAC | |
| pMT2-F | TTGCCTTTCTCTCCACAGGT | 3' end of synthetic intron, forward primer |
| pTRC99C-F | TTGCGCCGACATCATAAC | |
| mCherry-R | TTGGTCACCTTCAGCTTGG | 5' end of mCherry, reverse primer |
| DuetUP2 | TTGTACACGGCCGCATAATC | |
| TK-pA-R | TTGTCTCCTTCCGTGTTTCA | Thymidine kinase polyA, reverse primer |
| XEF1a | TTTCGCCCTAACTTCGTGAT | Xenopus EF1 alpha enhancer/promoter, forward primer |
| Bglob-intron-R | TTTGCCCCCTCCATATAACA | Rabbit beta-globin intron, reverse primer |
| P12584 | TTTTCAGTATCTACGAT | |
| Bglob-pA-R | TTTTGGCAGAGGGAAAAAGA | Rabbit beta-globin polyA region, reverse primer |
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