各种颜色荧光蛋白的最大激发波长和辐射波长

Protein
(Acronym)

Excitation
Maximum
(nm)

Emission
Maximum
(nm)

Molar
Extinction
Coefficient

Quantum
Yield

in vivo
Structure

Relative
Brightness
(% of EGFP)

GFP (wt)

395/475

509

21,000

0.77

Monomer*

48

绿色荧光蛋白

EGFP

484

507

56,000

0.60

Monomer*

100

Emerald

487

509

57,500

0.68

Monomer*

116

Superfolder GFP

485

510

83,300

0.65

Monomer*

160

Azami Green

492

505

55,000

0.74

Monomer

121

mWasabi

493

509

70,000

0.80

Monomer

167

TagGFP

482

505

58,200

0.59

Monomer*

110

TurboGFP

482

502

70,000

0.53

Dimer

102

AcGFP

480

505

50,000

0.55

Monomer*

82

ZsGreen

493

505

43,000

0.91

Tetramer

117

T-Sapphire

399

511

44,000

0.60

Monomer*

79

蓝色荧光蛋白

EBFP

383

445

29,000

0.31

Monomer*

27

EBFP2

383

448

32,000

0.56

Monomer*

53

Azurite

384

450

26,200

0.55

Monomer*

43

mTagBFP

399

456

52,000

0.63

Monomer

98

青色荧光蛋白

ECFP

439

476

32,500

0.40

Monomer*

39

mECFP

433

475

32,500

0.40

Monomer

39

Cerulean

433

475

43,000

0.62

Monomer*

79

CyPet

435

477

35,000

0.51

Monomer*

53

AmCyan1

458

489

44,000

0.24

Tetramer

31

Midori-Ishi Cyan

472

495

27,300

0.90

Dimer

73

TagCFP

458

480

37,000

0.57

Monomer

63

mTFP1 (Teal)

462

492

64,000

0.85

Monomer

162

黄色荧光蛋白

EYFP

514

527

83,400

0.61

Monomer*

151

Topaz

514

527

94,500

0.60

Monomer*

169

Venus

515

528

92,200

0.57

Monomer*

156

mCitrine

516

529

77,000

0.76

Monomer

174

YPet

517

530

104,000

0.77

Monomer*

238

TagYFP

508

524

64,000

0.60

Monomer

118

PhiYFP

525

537

124,000

0.39

Monomer*

144

ZsYellow1

529

539

20,200

0.42

Tetramer

25

mBanana

540

553

6,000

0.7

Monomer

13

橙色荧光蛋白

Kusabira Orange

548

559

51,600

0.60

Monomer

92

Kusabira Orange2

551

565

63,800

0.62

Monomer

118

mOrange

548

562

71,000

0.69

Monomer

146

mOrange2

549

565

58,000

0.60

Monomer

104

dTomato

554

581

69,000

0.69

Dimer

142

dTomato-Tandem

554

581

138,000

0.69

Monomer

283

TagRFP

555

584

100,000

0.48

Monomer

142

TagRFP-T

555

584

81,000

0.41

Monomer

99

DsRed

558

583

75,000

0.79

Tetramer

176

DsRed2

563

582

43,800

0.55

Tetramer

72

DsRed-Express (T1)

555

584

38,000

0.51

Tetramer

58

DsRed-Monomer

556

586

35,000

0.10

Monomer

10

mTangerine

568

585

38,000

0.30

Monomer

34

红色荧光蛋白

mRuby

558

605

112,000

0.35

Monomer

117

mApple

568

592

75,000

0.49

Monomer

109

mStrawberry

574

596

90,000

0.29

Monomer

78

AsRed2

576

592

56,200

0.05

Tetramer

8

mRFP1

584

607

50,000

0.25

Monomer

37

JRed

584

610

44,000

0.20

Dimer

26

mCherry

587

610

72,000

0.22

Monomer

47

HcRed1

588

618

20,000

0.015

Dimer

1

mRaspberry

598

625

86,000

0.15

Monomer

38

dKeima-Tandem

440

620

28,800

0.24

Monomer

21

HcRed-Tandem

590

637

160,000

0.04

Monomer

19

mPlum

590

649

41,000

0.10

Monomer

12

AQ143

595

655

90,000

0.04

Tetramer

11